解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose....
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0666-0
更新日期:2017-03-27 00:00:00
abstract:BACKGROUND:Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the mitochondrial thiol oxidase ERV1 gene was the most effective for high GSSG production in Saccharomyc...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0658-0
更新日期:2017-03-15 00:00:00
abstract:BACKGROUND:C4-dicarboxylic acids, including malic acid, fumaric acid and succinic acid, are valuable organic acids that can be produced and secreted by a number of microorganisms. Previous studies on organic acid production by Aspergillus carbonarius, which is capable of producing high amounts of citric acid from varie...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0660-6
更新日期:2017-03-14 00:00:00
abstract:BACKGROUND:Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity and efficacy, a variety of membrane receptors. Disulphide-reticulated peptides have evolved to display improved specificity, low immunogenicity and t...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0617-1
更新日期:2017-01-17 00:00:00
abstract:BACKGROUND:Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complica...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0620-6
更新日期:2017-01-03 00:00:00
abstract:BACKGROUND:Plasmid expression is a popular method in studies of MVA pathway for isoprenoid production in Escherichia coli. However, heterologous gene expression with plasmid is often not stable and might burden growth of host cells, decreases cell mass and product yield. In this study, MVA pathway was divided into thre...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0607-3
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Microbial genome editing is a powerful tool to modify chromosome in way of deletion, insertion or replacement, which is one of the most important techniques in metabolic engineering research. The emergence of CRISPR/Cas9 technique inspires various genomic editing methods. RESULTS:In this research, the goal ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0605-5
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0560-1
更新日期:2016-09-22 00:00:00
abstract:BACKGROUND:Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications deriv...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0545-0
更新日期:2016-08-24 00:00:00
abstract:BACKGROUND:Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0532-5
更新日期:2016-08-11 00:00:00
abstract:BACKGROUND:In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Alt...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0538-z
更新日期:2016-08-11 00:00:00
abstract:BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0530-7
更新日期:2016-08-02 00:00:00
abstract:BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0527-2
更新日期:2016-07-28 00:00:00
abstract:BACKGROUND:The biocontrol strain Pseudomonas chlororaphis GP72 isolated from the green pepper rhizosphere synthesizes three antifungal phenazine compounds, 2-Hydroxyphenazine (2-OH-PHZ), 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) and phenazine-1-carboxylic acid (PCA). PCA has been a commercialized antifungal pest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0529-0
更新日期:2016-07-28 00:00:00
abstract:BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0504-9
更新日期:2016-06-08 00:00:00
abstract:BACKGROUND:Streptomyces venezuelae ATCC 10712 produces antibiotics chloramphenicol (Cml) and jadomycin (Jad) in response to nutrient limitation and ethanol shock (ES), respectively. Biosynthesis of Cml and Jad was shown to be reciprocally regulated via the action of regulatory proteins JadR1 and JadR2 encoded by the ja...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0484-9
更新日期:2016-05-20 00:00:00
abstract:BACKGROUND:The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics and a work-intense s...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0486-7
更新日期:2016-05-20 00:00:00
abstract:BACKGROUND:Under aerobic conditions, acetic acid is the major byproduct produced by E. coli during the fermentation. And acetic acid is detrimental to cell growth as it destroys transmembrane pH gradients. Hence, how to reduce the production of acetic acid and how to utilize it as a feedstock are of intriguing interest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0475-x
更新日期:2016-05-05 00:00:00
abstract:BACKGROUND:The Gram-positive bacterium Bacillus subtilis has been widely used as a cell factory for the production of proteins due to its generally regarded as safe (GRAS) nature and secretion capability. Of the known secretory pathways in B. subtilis, the majority of proteins are exported from the cytoplasm by Sec pat...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0469-8
更新日期:2016-04-28 00:00:00
abstract:BACKGROUND:Understanding the metabolic mechanism of sterols to produce valuable steroid intermediates in mycobacterium by a noncoding small RNA (sRNA) view is still limited. In the work, RNA-seq was implemented to investigate the noncoding transcriptome of Mycobacterium neoaurum (Mn) in the transformation process of st...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0462-2
更新日期:2016-04-25 00:00:00
abstract:BACKGROUND:Anaerobic digesters become unstable when operated at a high organi c loading rate (OLR). Investigating the microbial community response to OLR disturbance is helpful for achieving efficient and stable process operation. However, previous studies have only focused on community succession during different proc...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0466-y
更新日期:2016-04-25 00:00:00
abstract:BACKGROUND:Intracellular metabolism of glucocorticoid hormones plays an important role in the pathogenesis of metabolic syndrome and regulates, among many physiological processes, collagen metabolism in skin. At the peripheral level the concentration of active glucocorticoids is mainly regulated by the 11β-hydroxystero...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0450-6
更新日期:2016-03-15 00:00:00
abstract:BACKGROUND:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0446-2
更新日期:2016-03-04 00:00:00
abstract:BACKGROUND:Despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. Shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. Thu...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0444-4
更新日期:2016-02-24 00:00:00
abstract:BACKGROUND:(2S)-Pinocembrin is a chiral flavanone with versatile pharmacological and biological activities. Its health-promoting effects have spurred on research effects on the microbial production of (2S)-pinocembrin. However, an often-overlooked salient feature in the analysis of microbial (2S)-pinocembrin is its chi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0424-8
更新日期:2016-02-04 00:00:00
abstract:BACKGROUND:Protoilludene is a valuable sesquiterpene and serves as a precursor for several medicinal compounds and antimicrobial chemicals. It can be synthesized by heterologous expression of protoilludene synthase in Escherichia coli with overexpression of mevalonate (MVA) or methylerythritol-phosphate (MEP) pathway, ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0409-7
更新日期:2016-01-19 00:00:00
abstract:BACKGROUND:Catalase (EC 1.11.1.6) is one of the important industrial enzyme employed in diagnostic and analytical methods in the form of biomarkers and biosensors in addition to their enormous applications in textile, paper, food and pharmaceutical sectors. The present study demonstrates the utility of a newly isolated...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0410-1
更新日期:2016-01-14 00:00:00
abstract:BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0381-7
更新日期:2015-11-26 00:00:00
abstract:BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0377-3
更新日期:2015-11-25 00:00:00
abstract:BACKGROUND:Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. METHODS:We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxy...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0378-2
更新日期:2015-11-25 00:00:00
abstract:BACKGROUND:Phasin (PhaP), a kind of polyhydroxyalkanoate (PHA) granule-associated proteins, has a role in controlling the properties of PHA granules surface, and is thought to have influence on PHA biosynthesis in PHA-producing bacteria. This study focused on the phaP1(Re) locus in Ralstonia eutropha as a site of chrom...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0380-8
更新日期:2015-11-23 00:00:00
abstract:BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0368-4
更新日期:2015-11-05 00:00:00
abstract:BACKGROUND:Rhodosporidium and Rhodotorula are two genera of oleaginous red yeast with great potential for industrial biotechnology. To date, there is no effective method for inducible expression of proteins and RNAs in these hosts. RESULTS:We have developed a luciferase gene reporter assay based on a new codon-optimiz...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0357-7
更新日期:2015-10-26 00:00:00
abstract:BACKGROUND:Calreticulin (CRT) resides in the endoplasmic reticulum (ER) and functions to chaperone proteins, ensuring proper folding, and intracellular Ca(2+) homeostasis. Emerging evidence shows that CRT is a multifunctional protein with significant roles in physiological and pathological processes with presence both ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0356-8
更新日期:2015-10-15 00:00:00
abstract:BACKGROUND:Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. RESULTS:In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-produci...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0329-y
更新日期:2015-09-17 00:00:00
abstract:BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0319-0
更新日期:2015-09-15 00:00:00
abstract:BACKGROUND:4-Hydroxycinnamaldehydes are important intermediates in several secondary metabolism pathways, including those involved in the biosynthesis of phenolic acids, flavonoids, terpenoids and monolignols. They are also involved in the biosynthesis and degradation of lignins, which are important limiting factors du...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0309-2
更新日期:2015-08-12 00:00:00
abstract:BACKGROUND:Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry to develop new molecules using structure-based drug design studies. Specifically, membrane-bound catechol-O-methyltransferase (MBCOMT) is an...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0304-7
更新日期:2015-08-07 00:00:00
abstract:BACKGROUND:Cholesterol, the precursor of all steroid hormones, is the most abundant steroid in vertebrates and exhibits highly hydrophobic properties, rendering it a difficult substrate for aqueous microbial biotransformations. In the present study, we developed a Bacillus megaterium based whole-cell system that allows...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0300-y
更新日期:2015-07-29 00:00:00
abstract:BACKGROUND:Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of medical interest, as it is "Generally Recognized As Safe". Furthermore, as L. lactis naturally secretes only one major endoge...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0271-z
更新日期:2015-07-16 00:00:00